Bayern defender Daniel van Buyten will miss the trip to Spain because of personal reasons.
"The team left on Tuesday midday without Van Buyten for the match against Barcelona," the club said on their website.
Van Buyten is the second central defender to be ruled out, after Brazilian Lucio picked up a muscle injury in Bayern's 5-1 defeat against Wolfsburg on Saturday and will be out for several days.
Coach Juergen Klinsmann is expected to rely on Martin Demichelis and 19-year-old Brazilian Breno to harness the Spaniards' pace.
He also brought in Holger Badstuber from the reserve team."We are going to have to be at our very highest level to score goals and we know that at this level your mistakes are punished ruthlessly, especially against a team like Bayern."